網站地圖 聯系我們搜索內部網English | 中國科學院
首頁 所況簡介 機構設置 科研隊伍 科學研究平臺 院地合作 黨群園地 國際交流 博士后 研究生教育 信息公開
科研進展
成果報道
最新重要論文(影響因子PNAS及以上)
發表論文數據庫
所級學術報告
科學成果
發表論文
專著
專利
獲獎
專題
所史叢書
所慶專輯
建所50周年畫冊
現在位置:首頁 > 科研進展 > 最新重要論文(影響因子PNAS及以上)
Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States,Mol Cell, 24 Oct 2019
2019-10-24 | 【     】【打印】【關閉

Molecular Cell24 October, 2019,DOI:https://doi.org/10.1016/j.molcel.2019.09.025

Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States

Wei Sun, Jing Yang, Zhi Cheng, Nadia Amrani, Chao Liu, Kangkang Wang, Raed Ibraheim, Alireza Edraki, Xue Huang, Min Wang, Jiuyu Wang, Liang Liu, Gang Sheng, Yanhua Yang, Jizhong Lou, Erik J. Sontheimer, Yanli Wang

Summary

High-resolution Cas9 structures have yet to reveal catalytic conformations due to HNH nuclease domain positioning away from the cleavage site. Nme1Cas9 and Nme2Cas9 are compact nucleases for in vivo genome editing. Here, we report structures of meningococcal Cas9 homologs in complex with sgRNA, dsDNA, or the AcrIIC3 anti-CRISPR protein. DNA-bound structures represent an early step of target recognition, a later HNH pre-catalytic state, the HNH catalytic state, and a cleaved-target-DNA-bound state. In the HNH catalytic state of Nme1Cas9, the active site is seen poised at the scissile phosphodiester linkage of the target strand, providing a high-resolution view of the active conformation. The HNH active conformation activates the RuvC domain. Our structures explain how Nme1Cas9 and Nme2Cas9 read distinct PAM sequences and how AcrIIC3 inhibits Nme1Cas9 activity. These structures provide insights into Cas9 domain rearrangements, guide-target engagement, cleavage mechanism, and anti-CRISPR inhibition, facilitating the optimization of these genome-editing platforms.

文章鏈接:https://www.cell.com/molecular-cell/fulltext/S1097-2765(19)30730-0

相關報道:http://www.978858.live/kyjz/zxdt/201910/t20191025_5412332.html

 

 

評 論
          
版權所有:中國科學院生物物理研究所     京ICP備05002792號 京公網安備 110402500011 號
地址:北京市朝陽區大屯路15號(100101) 電話:010-64889872
意見反饋聯系人:侯文茹 電子郵件:[email protected]
彩票开奖直播 电脑单机版麻将游戏 微信股票群可靠吗 pc蛋蛋走势图分析 贵州捉鸡麻将规则 街机万人捕鱼游戏 喜乐彩开奖信息 雀友会广东潮汕麻将 全民捕鱼兑换码 金7乐彩票今日开奖结果 经典老版单机麻将旧版